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INS-1 832/3 SNAP/FLAG-hGLP-1R WT and V229A cells were generated in house from INS-1 832/3 GLP-1R KO cells following transfection with pSNAP/FLAG-hGLP-1R (Cisbio) or pSNAP/FLAG-hGLP-1R-V229A (generated in house by site-directed mutagenesis, see below), followed by selection with 1 mg/mL G418 sulphate (A1720, Sigma Aldrich)

This increases the amount of serotonin available in the brain, which helps improve mood and reduce anxiety