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K-means clustering of amplicon sequence variant data confirmed that 5-Amino-1MQ treatment generated a distinct cecal microbiome profile from all other treatment groups including diet-switched vehicle controls indicating that NNMT inhibition produces microbiome changes that are separable from those produced by dietary composition alone

The authors particularly pointed to reductions in two carnitines: oleyllcarnitine (C18:1) and linoleyllcarnitine (C18:2), which were highly correlated with fatigue levels in ME/CFS