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PIP 3 in turn activates the enzyme, PIP 3 -dependent kinase 1, (PDPK1)

Co-culture of HUVECs and THP-1 cells When the HUVEC confluency met approximately 6070%, the conditioned medium collected from THP-1 cell supernatants stimulated by hypoxia intervention and ECM was added into 6-well plates at a ratio of 1:1 and incubated for 24 h to simulate cell co-culture

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