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When IGF-1 axis is activated, multiple signaling pathways corresponding to its kinase domain are also activated, including PI3K/Akt pathway and Raf/MEK/ERK level linkage pathway, which can ultimately reduce IR and prevent cell apoptosis (58, 59), thereby improving NAFLD

Furthermore, FGF21 demonstrates protective effects against pancreatic fibrosis and inflammation

, downregulation

In that regard, NKCC2 has been detected in other organs at much lower levels than those found in the kidneys, including small intestines (Xue et al., 2009) and hypothalamic regions of the brain (Konopacka et al., 2015)