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The key is a thoughtful transition that prioritizes gradual dosing, symptom monitoring, and individualized care

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This can be risky if you are standing up quickly, exercising, driving, drinking in hot weather, or combining alcohol with other medications that cause drowsiness

The next day, sections were rinsed, incubated in secondary antibody in blocking solution for 2 h at room temperature on rotation and then rinsed and counterstained with Hoechst 33342 (1:2000 in PBS, Fisher #H3570) before being mounted on SuperFrost Plus slides (Fisher #12-550-15), dried and coverslipped with Prolong Gold Antifade mounting medium (Fisher #P36934)