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There are a number of variations in these procedures, including the use of provocative chemical agents such as sodium lauryl sulfate (Kligman, 1966c), special skin preparation such as stripping (Spier & Sixt, 1955) or freezing (Epstein et al., 1963), special patches (Magnusson & Hersle, 1965), high concentrations at induction (Marzulli & Maibach, 1974), and 25-200 test subjects (Draize et al., 1944