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Carbonic anhydrase 9 (CA9) expression in non-small-cell lung cancer: correlation with regulatory FOXP3+T-cell tumour stroma infiltration

qPCR was performed with an initial denaturation at 95 C for 30 s, 40 amplification cycles (95 C for 10 s, 65 C for 30 s), followed by melting curve acquisition (95 C for 15 s, 65 C for 60 s, 97 C for 1 s) and cooling at 37 C for 30 s

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