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Next, the sections were incubated with primary antibodies, including rabbit anti-ionized calcium-binding adapter molecule-1 (Iba1) (1:1000, 019-19741, Wako), mouse anti-glial fibrillary acidic protein (GFAP) (1:1000, MAB360, Millipore), rabbit anti-sex determining region Y-Box 2 (Sox2) (1:1000, AB5603, Millipore), rabbit anti-doublecortin (DCX) (1:1000, 4604S, Cell Signaling Technology), mouse anti-BrdU (1:500, MAB3424, Millipore) and rabbit anti-Prox1 (1:500, 925202, Biolegend), at 4 C overnight