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Likewise, producing soluble pHLA-I complexes by in vitro refolding one peptide at a time hinders library-scale screening, as even a modest screen of 50 peptides for a single HLA-I allotype could take months to generate [16]

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The relation between TMAO and chronic disease can be ambiguous, involving kidney function [79], disturbed gut-blood barrier [83], or flavin-containing monooxygenase 3 genotype [84]