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Rats had ad libitum access to water and SD (Picolab, rodent diet 5053) until 10 months of age in which they were divided into weight-matched groups within each sex, and fed a SD (Picolab, rodent diet 5053) or WD (Research Diets (RD) Western Diet, D12079B, Research Diets Inc., New Brunswick, NJ, USA) for the following 8 weeks of the experiment as described below and demonstrated in Figure 1A

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In many cases, switching from oral to intramuscular B12 circumvents absorption issues entirely, providing reliable therapeutic levels regardless of gastrointestinal factors or drug interactions

However, once it was discovered that our body can make its own inositol, it was no longer considered a true vitamin