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After removal of non-adherent cells by washing the plates, monocytes were differentiated into macrophages in RPMI medium (Gibco, Grand Island, NY, USA) supplemented with 10% FCS (Gibco) plus antibiotics and antimycotics (100 U/ml penicillin, 100 g/ml streptomycin, and 25 g/ml amphotericin

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Hypothyroidism reduces the expression of tight junction proteins, increasing permeability and facilitating immune cell infiltration (119)