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At an OD 600 of 0.60.7, expression was induced with 1 mM IPTG and the culture was continued up to an OD 600 of 3.0 where the cells were harvested at the end of the exponential growth phase by centrifugation (10 min, 3,220 g , 4C)

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according to data retrieved from the Swedish National Diabetes Register, the adjusted hazards ratio (HR) for AF in T1DM patients is 1.13 (95% CI 1.011.25, P = 0.029) in men and 1.50 (95% CI 1.301.72, P 27 kg/m 2 (2)