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Reconstitution & Handling Guidelines Because of the highly concentrated peptide mass (80mg) and the inclusion of the copper ion complex, reconstitution must be handled methodically to avoid shearing the peptide bonds: Recommended Solvent: Bacteriostatic Reconstitution Solution (0.9% Benzyl Alcohol)

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There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively-charged Copper and peptide to the positively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes

Cycling preserves the peptide's effectiveness and gives the hypothalamic-pituitary axis time to reset