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Essential preparation steps include: Hand hygiene wash hands thoroughly with soap and water or use alcohol-based hand sanitiser Gather supplies B12 ampoule, sterile syringe and needle, alcohol wipes, sharps container, and cotton wool or gauze Check the medication verify the correct drug, strength, and expiry date

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Purified basophils pre-incubated with luteolin strongly inhibited IL-4 synthesis (IC 50 = 2.6 M)

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