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The flow rate was maintained at 0.15 ml min 1 with the following linear gradient: 023 min from 95% buffer B to 5% buffer B, 2325 min hold at 5% buffer B, 2525.5 min transition to 95% buffer B at 0.20 ml min 1 , 25.532.5 min hold at 95% buffer B and 32.533 min at 95% buffer B with a 0.15 ml min 1 flow rate

This activates the longevity proteins Sirtuins, promotes cell regeneration, and repairs damaged DNA, thereby slowing down skin aging, reducing fine lines and wrinkles, and improving skin elasticity and firmness