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Research areas where laboratories buy NAD+ for in vitro work include dehydrogenase enzyme kinetics read via the 340 nm NAD+/NADH absorbance shift, sirtuin and PARP activity assays, cell-culture redox studies measuring the NAD+/NADH ratio, mitochondrial bioenergetics, and comparative studies of NAD+ replenishment versus precursor supplementation (NMN, NR, niacin)