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In our experiment, some indicators in the blood gas analysis of the plateau animal model changed when the simulated altitude was 6000 m

The reason is simple: those guides are written by people who've read other guides, not by researchers who've troubleshot failed protocols and traced the failure back to the reconstitution step

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Primary antibodies against FOXO4 (Cell Signaling Technology 9472) and TP53BP1 (BD Biosciences 612523) were diluted in 1% BSA in PBST and incubated with the cells overnight at 4C