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Patients usually experience: Sudden Vision Loss: Sudden, painless vision loss in one eye that occurs upon waking up or is noticed within the first few hours of the day Visual Filed Defects : Specific visual field defects, most commonly affecting the lower part of the visual field Optic Disc Swelling : Swelling of the optic disc and visible bleeding during an eye examination Pupil Defect : A relative defect in pupil response in the affected eye Dark or Gray Spot: The vision loss from NAION often consists of a gray or dark spot that does not move or change or a severe blur

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The control can either be lysate from separately transformed cells that express GST (not the bait fusion protein) or lysate from non-transformed cells to which GST is added