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Calculation of the degree of oxidation (OxD roGFP2) was performed according to equation 1: For plate-reader experiments, yeast cultures were grown to late logarithmic phase (OD 600 = 34) in liquid HC medium lacking histidine for plasmid retention

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Glutathione peroxidases 4 (GPX4) is a selenocysteine-containing, and GSH-dependent monomeric enzyme (Kelner and Montoya, 1998