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available only through 503A compounding pharmacies under physician supervision Typical research dose range / 200-800 mcg/day subcutaneously, cycled 4-8 weeks Known lethal dose (LD50) / Not established in any species studied to date Rodent acute toxicity ceiling / No mortality observed at doses exceeding 10 mg/kg intraperitoneally Most reported excess-dose symptoms / Nausea, lightheadedness, flushing, injection-site irritation Organ toxicity at supratherapeutic doses / Not demonstrated in published preclinical literature Antidote availability / No specific antidote exists

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Preparative cultures (1 l) for each transformed strain were incubated with shaking at 37C in lysogeny broth (LB) supplemented with 5 mM AcK (A4021-5G Sigma), 10 M sodium selenite (10102-18-8, AlfaAesar), and appropriate antibiotics (100 g/ ml ampicillin (BP1760-25, Fisher) for pET and 34 g/ ml chloramphenicol (02930-100G, Ampresco) for pTech)