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It starts with the design principles of peptide sequences, after that the screening for different stapled positions, reaction types, and properties of staples, and then the characterization of in vitro bioactivity including targeting specificity, binding affinity of target proteins, and ability to resist protein hydrolysis, and next the cell permeability required for targeting intracellular PPIs, finally the optimization of stapling such as the introduction of stapling with different properties or functions

The most common adverse reactions with dulaglutide are gastrointestinal side effects such as nausea, vomiting, and diarrhea

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