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Cytokines Measurement The levels of TNF- and IL-1 in serum and peritoneal lavage fluid collected at 24 h post CLP induction were quantified by Enzime-Linked Immunosorbent Assay (ELISA) using an appropriate commercial kit (R&D Systems, Minneapolis, Canada) according to the manufacturer's instructions

Maintain sterility - Using sterile solvents and technique prevents contamination

200 mg of MLE - the same amount in one dose of Renutrin decreased glucose by -30% and insulin by 31% in healthy adults

The most important immediate objective is accurate diagnosis and risk control to reduce the chance of additional injury, including involvement of the other eye