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Techniques like ion-pairing reversed-phase liquid chromatography (IP-RPLC) are widely used to monitor peptide critical quality attributes (CQAs), with hyphenated LC-MS approaches playing a key role in enhancing sensitivity and selectivity, enabling quantification of low-level impurities

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FIGURE 12 3.6 Comparative estimation of free energy of binding between SR18GLP-1R and GLP-1GLP-1R complexes To further understand the energetic contribution of the crucial residues of GLP-1R toward SR18 compared to GLP-1, the MD trajectories were subjected to gmx_MMPBSA calculations