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Briefly, the head was decapitated by cutting posterior to the ears, and a midline skin incision was made caudal to the sagittal suture, with care taken not to cut through the brain
Consequently , applications include: Bone Research: Osteoblast differentiation, mineralization markers, osteocalcin expression, alkaline phosphatase activity, collagen type I synthesis, and bone density assessment Tendon and Ligament Studies: Fibroblast proliferation, collagen synthesis (types I, III), biomechanical properties, and healing process characterization Cartilage Investigation: Chondrocyte function, proteoglycan synthesis, aggrecan expression, collagen type II, and extracellular matrix maintenance Furthermore , a 2026 PMC review highlights therapeutic peptides, including GH secretagogues, as emerging adjuncts in orthopedic injury management
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3) reviews, systematic reviews, meta-analyses, and letters
