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These effects are transient in many cases, declining after 1 to 2 weeks as tolerance develops, though individual variation is significant

After treated with test compounds for 15 hours, harvested in 5 ml Dulbecco s modified medium containing Deep Red Reagent (5 M) (Molecular Probes, Invitrogen) and incubated for 30 min at 37C in a tissue culture incubator

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