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The human airway receives many insults from pathogens, pollen, dust, etc., every day that can contribute to illness, and people who have predispositions to deficiencies in mucus clearance are far more likely to experience an insult that tips their airway from maintaining homeostasis to cycling indefinitely between mucus hypersecretion, dehydration, plugging, and infection

Homogenates were centrifuged at 800 g for 10 min at 4 C, and the supernatant was sonicated on ice for 20 s before protein quantification (BCA protein assay, Pierce, Rockford, IL, USA)