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After the cells reached ~ 80% confluence under normal culture conditions, the medium was changed to glucose-free DMEM (Gibco) and the dish was placed in an anaerobic chamber (5% CO2, 95% N2) to induce OGD-related cell injury

Liu Z, Zhang Y, Youn JY, Zhang Y, Makino A, Yuan JXJ, Cai H

Interestingly, mice exposed to 4EPS exhibited anxiety-like behaviors, and treatments that promote oligodendrocyte differentiation were able to prevent the behavioral effects caused by 4EPS (Needham et al., 2022)

Effective fat burning occurs through many different pathways, but one of the most efficient ways is to tap into stored fatty acids and dietary fat as an energy source