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Functional in vitro characterization of slco1b1 variants and simulation of the clinical pharmacokinetic impact of impaired oatp1b1 function

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Lentivirus was produced in 293T cells using Lipofectamine 2000 (9 g packaging plasmid psPAX2 (Addgene 12260), 0.9 g envelope plasmid pCAG-VSVG (Addgene 35616), 9 g scramble control or shLDHB vectors (Origene TL311768) in 225 L Opti-MEM (Thermo Fischer 31985062) mixed with 90 L Opti-MEM containing 54 L Lipofectamine 2000, 10-cm dish), the medium was changed after 18 h of incubation, then the virus was collected 24 and 48 h later