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The 1998 Raun characterisation paper documented that ipamorelin stimulated GH release in pigs and rats with potency comparable to GHRP-6 but without the prolactin, ACTH, cortisol, or aldosterone elevations characteristic of earlier GHRPs (PMID 9849822)

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50 L of the supernatant was transferred to a well on the assay plate and 200 L of assay buffer was added to each well