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The TME is characterized by nutrient deprivation, hypoxia and an acidic milieu, driven by aberrant tumor vasculature and the Warburg effect (a preference for aerobic glycolysis even in the presence of oxygen), which collectively facilitate immune evasion by tumor cells (88,89)

Whilst the 3T3-L1 mouse pre-adipocyte cell line is extensively utilised for identifying adipogenic chemicals, and it is easier to use a cell line, rather than primary cells, the species relevance with respect to induction of human adipogenesis is weaker

Fat loss benefits may take longer with consistent sessions

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