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For unsecreted and DTC-associated basement membrane COL IV measurements, with the same images used for DTC-AC quantification, 3D models were generated based on the red EMB-9::mCherry marker using identical settings that allowed for consistent and specific labelling of the DTC intracellular punctuate signals that are referred to as blobs in the manuscript

: Orforglipron (Non-peptide small molecule): Activates GLP-1 receptors without peptide degradation

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