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Validation experiment based on parallel reaction monitoring (PRM) To further validate our proteomics data, we selected proteins related to lipid synthesis (Cd36, Fabp2, Fabp4, Plin2, Plin4, and Plin5), fatty acid oxidation (FAO) (Cpt1a, Acaa1b, and Cyp4a10), the mitophagy-lysosome pathway (Bnip3, Sqstm1, Optn, Map1lc3b, Lamp1, Lamp2, and Atp6v0d1), cholesterol metabolism (Abdg5, Abcg8, Cyp7a1, and Hnf4a), and the oxidative stress response (Gstm2, Hmox1, and Hspb1) for targeted proteomics analysis

This aligns with the moderate behavioral improvements seen in social interactions, suggesting that oxidative stress and excitatory overactivity continued to drive damage despite partial restoration of GABAergic function