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3.1.7 Voltage-dependent anion channels in ferroptosis and oxidative stress Voltage-dependent anion channels (VDACs), membrane pore proteins located in the outer mitochondrial membrane, facilitate ion and metabolite transport in eukaryotic cells (39)

The purified recombinant MhTyrA p Q227E enzyme (Figure S3) showed decreased PDH activity (Figure 4D) with a substantial gain of ADH activity (Figure 4C, Table 1) without altering cofactor preference (Figure S4)

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Results Overall structures of CCK1R and CCK2R signaling complexes For structural studies with different transducer-coupled CCKRs (CCK1RG s and CCK2RG q ), human CCK1R and CCK2R with 15-residue depletion at the C-terminus were subcloned into pFastBac1 vector with an N-terminal Flag-tag, along with a LgBiT and double maltose-binding protein (MBP) affinity tag at the C-terminus to facilitate the protein expression and purification 42