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The cells (3 10 6 cells/mL) were transfected with 1 g of plasmid DNA per mL of cell culture using ExpiFectamine 293 Reagent (Gibco)

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Notably, a 2020 clinical trial involving patients with nonalcoholic steatohepatitis (NASH) revealed a 15% incidence of tumors (benign, malignant, or unspecified) (35/239) among those administered semaglutide, compared to 8% (6/80) in the control group [108]

Studies suggest that GLP-1RAs reduce inflammation by modulating immune cell activity, increasing anti-inflammatory cytokine production, shifting macrophage polarization toward an anti-inflammatory phenotype, and enhancing regulatory T-cell function to maintain immune homeostasis