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Other studies have shown that perinatal administration of 2 mM quinine on e17 affected the expression level of gustatory (palate) and extra-gustatory (duodenum) mRNA for the three bitter taste receptors ggTas2r1, ggTas2r2, and ggTas2r7 and their signaling components alpha-gustducin, phospholipase, and transient receptor potential melastatin 5 (163)

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Briefly, samples were lysed in RIPA Plus lysis buffer (50 mM Tris, 1% IGEPAL, 0.5% sodium deoxycholate, 0.1% SDS, 150 mM NaCl, 2 mM EDTA, 50 mM NaF, protease inhibitors)