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Ethical approval for the study was obtained from the Pak-Austria Institutional Research Ethics Committee (Approval No

10.1002/dmrr.3173 Summary Keywords metformin, polycystic ovary syndrome, infertility, mechanism, drug therapy Citation Zeng W, Luo Y, Ou J, Gan D, Huang M, Tomlinson B and Jiang Y (2025) Metformin in polycystic ovary syndrome: unraveling multi-stage therapeutic mechanisms from puberty to long-term health outcomes

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Observed mechanisms include direct neuronal damage, disruption of key neurotransmitter pathways such as AChE activity, induction of cellular stress responses, and alterations in both locomotor and spontaneous activity patterns, particularly in Drosophila