Thrombus formation and the subsequent activation of the coagulation cascade with final generation of fibrin is facilitated by the mCRP-mediated enhancement of tissue factor on the endothelial cell surface, platelet aggregation and thrombus growth (207, 208) (Figure 7)
2.8.2 Data analysis of solute carrier (SLC) substrate and inhibition study Regarding the substrate assay, the concentrations of the compounds or probe substrates in the cell lysate were determined using LC-MS and used to calculate the uptake rate (pmol/mg/min) using Equation 3.where, C lysate , P, and T represent the concentrations detected in the cell lysate (pmol), protein concentration in the cells (mg), and incubation time (min), respectively
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3.3.3 Bile acids Bile acids are synthesised in the hepatocyte by a complex multienzyme process (with at least 17 steps) from cholesterol