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TMG (betaine) supports methylation by donating methyl groups, which helps maintain normal homocysteine metabolism and cellular balance

In most cases, where the fusion protein is initially observed primarily in inclusion bodies, it is desirable to modify culture conditionssuch as lowering growth temperature, increasing aeration, or altering induction conditions toattempt to obtain soluble protein, rather than to solubilize inclusion bodies with denaturants and refold the protein after extraction

Wang Y, Xu G, Ren Z et al (2007) Mineralizer-assisted hydrothermal synthesis and characterization of BiFeO3 nanoparticles