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Most strikingly, late-life-initiated intermittent treatmentstarted at 23.5 months, the murine equivalent of late human lifeincreased physical capacity and healthspan

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In 2020, the same group of researchers conducted another study that aimed to evaluate the anticonvulsant effects of AC and investigate its underlying mechanisms

At the end point, cells were collected by trypsinization (the culture medium and PBS washes were collected and re-added to each sample) and washed once with assay buffer (10 mM HEPES, 140 mM NaCl and 3.3 mM CaCl 2 , pH 7.4)