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75 mM Tris pH 8, 75 mM NaCl, 1 mM EDTA, 1 complete miniprotease inhibitor cocktail tablet per 10 ml, 5 mM sodium fluoride, 1 mM sodium orthovanadate, 5 mM -glycerol phosphate) and dropped in droplets out of pipette into liquid nitrogen

5.3 Safety and off-target effects Modulating the immune system and mitochondria can have unintended consequences

Mutation of the TERT promoter, switch to active chromatin, and monoallelic TERT expression in multiple cancers

Functional enrichment analysis of differentially expressed proteins To elucidate the biological implications of the differentially expressed proteins, Gene Ontology (GO) enrichment analysis was performed to identify significantly enriched biological processes, cellular components, and molecular functions associated with proteins upregulated (Fig