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This scheme proposes the following steps: (i) a one electron reduction of lucigenin (L) under the influence of cytochrome P450-reductase and NADPH to generate a radical species (LH + ), (ii) a reaction between the latter and ground state oxygen to produce O 2 - and recycle the lucigenin back to its native state (L), (iii) and finally a reaction between LH + and O 2 - , generated as a result of the redox cycling of lucigenin, to produce a dioxetane that, in turn, decomposes with the generation of chemiluminescence (Fig