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The sections were subsequently hematoxylin counterstained, dehydrated through graded ethanol, cleared with xylene, and mounted for microscopic analysis

Treatment duration proves critical variable where longer courses create more durable changes - users completing 2-3 month treatments typically maintain 20-40% of peak benefits long-term, 4-6 month treatments sustain 40-60% of improvements, and 6-12+ month courses preserve 50-70% of maximum benefits potentially indefinitely

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It breaks down damaged, disorganized collagen and replaces it with new, properly structured fibers