Roalf DR, Nanga RPR, Rupert PE, Hariharan H, Quarmley M, Calkins ME, et al
Disulfide linkage reduction was performed, then samples were incubated in blocking solution (PBS with 1% (w/v) bovine serum albumin, 0.5% (v/v) Triton X-100, 1 mM EDTA) for 40 minutes at RT, followed by overnight incubation at 4 with rabbit -PGL-1 (1:100, gift from Susan Strome, University of California, Santa Cruz) diluted in blocking solution
Curiously, some bariatric RYGB patients experience up to a 10-fold increase in post-meal active GLP-1 plasmatic levels (from fasting 5 pM to post-prandial 3065 pM) (146), and have a 2- to 3-fold higher glucose-stimulated Insulin secretion (147), which in some diabetic patients results in GLP-1-mediated hyperinsulinemic hypoglycaemia that requires GLP-1 antagonism or surgical reversal of the intestinal anatomy (148)
The solution is straightforward but requires intentional planning: prioritize protein intake at every meal and combine it with resistance training
