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Antioxidative Enzyme Activity Assay in the Frontal Cortex and Hippocampus Glutathione Peroxidase Brain tissue was homogenized in 7 mL of cold buffer (50 mM TrisHCl, pH 7.5, 5 mM EDTA, and 1 mM DTT) per gram tissue and centrifuged (10,000 g for 15 min at 4 C)

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GSH can also be used as a source of sulfur and cysteine

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