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insert needle at 4590 angle (depending on needle length and fat thickness) into subcutaneous tissue [7] [8]

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To analyze the data, we employed partial least squares discriminant analysis (PLS-DA), a supervised method that optimizes group separation and aids in building a novel machine-learning model for pathogen identification

Eluates were dried and then resolubilized in 100 L of 10% ACN prior to LC-MS/MS analysis