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Reagan-Shaw 2007)

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The chromatographic gradient was set as follows: 0 minutes at 30% B, 2.00 minutes at 43% B, 2.1 minutes at 55% B, 12.00 minutes at 65% B, 18.00 minutes at 85% B, 20.00 minutes at 100% B, maintained at 100% B until 25.00 minutes, then returned to 30% B at 25.1 minutes and held until 30.00 minutes