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While the endosome-dependent pathway is acknowledged as a principal mechanism for Exos biogenesis, certain investigations indicate that direct budding from the plasma membrane also contributes to a considerable fraction of Exos formation [47, 66, 67]

Consistently, in vitro data, including our findings, show that bacterially purified monomeric Syn WT also resists aggregation compared to PD-specific PTM-Syn variants 33,44,49 and remains inert unless its hydrophobic core is exposed 15,68