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In order to detect the effect of Epitalon on the membrane potential of aging oocytes, JC-1 was used to analyze the membrane potential, and the ratio of red/green fluorescence of oocytes was evaluated to reflect mitochondrial function in control, aging, and 0.1mM Epitalon groups

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Relative fold change in cell number of the indicated HEK-293T SLC25A39 knockout cells expressing vector or SLC25A39 cDNA transduced with the indicated sgRNAs

The Students t test was used to analyse differences between paired data, whereas one-way ANOVA was used to analyse multiple comparisons