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Sampling Rats were euthanized after 14 days by cervical dislocation following anaesthesia to obtain lung tissue samples that were split into two portions

We identified the candidate gr mutant firstly by observing fragment size in a PCR analysis, and all observed PCR fragments were in accordance with the predicted sizes as described in the Materials and Methods section (Figure 6A)

Non-haem iron: a dietary iron found in vegetables, dairy, eggs, and fortified products