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The lyophilized cake should look intact and dry

The blocks were cut into 1 mm cubes and washed in double distilled water (ddH 2 O) for 10 min before being processed into resin in a rotary mixer at room temperature by the following steps: Post-fixed for 1 h in 2% (w/v) osmium tetroxide in ddH 2 O, 10 min wash in ddH 2 O (x3), block stained for 1 h in 2% uranium acetate in ddH 2 O, 10 min wash in ddH 2 O (x3), 15 min in 50% (v/v) propan-2-ol (IPA) in ddH 2 O, 15 min in 70% (v/v) IPA in ddH 2 O, 15 min in 90% (v/v) IPA in ddH 2 O, 15 min in IPA (2x), 30 min in 50% (v/v) hard grade TAAB Embedding Resin (TER) in IPA, 1 h in TER (4x)

At a "1 mg retatrutide + 0.2 mg cagrilintide" combined dose: 0.16 mL = 16 units

Then, 95 L supernatant was transferred to a labeled 1.5-mL microcentrifuge tube, and 5 L of an internal standard of Sph/Cer was added